|
KMID : 0385020000160020159
|
|
Korean Journal of Laboratory Animal Science
2000 Volume.16 No. 2 p.159 ~ p.167
|
|
|
Studies on the cryopreservation of semen for production of SPF rabbit 1. Effects of cooling rate and saccharides on viability and acrosome integrity of rabbit spermatozoa
|
|
|
|
|
Abstract
|
|
|
This experiment were carried out to investigate the effects of cooling rates at which the temperature fell down to 5℃ from 25℃ and combinations of saccharide with cell penetrating or non-penetrating cryoprotective in the egg-yolk extenders on the viability and acrosome integrity of the post-thawed rabbit sperm.
The results obtained in this experiment are summarized as follows:
1. Viability of the post-thawed sperm increased at the rapid cooling rates, with cell penetrating as cryoprotective(p$lt;0.05); on the other hand, sperm viability was shown high at slow cooling rates, with cell non-penetrating as cryoprotective(p$lt;0.05).
2. Rates of normal acrosome of post-thawed sperm were higher when methyl cellulose among cell non-penetrating cryoprotectants was added to the extender than when cell penetrating cryoprotectives was added to it.
3. Viability of sperm was higher when both cell penetrating and non penetrating cryoprotectives were added to the extender containing egg-yolk than when cell penetrating or non-penetrating cryoprotectants alone(P$lt;0.05) were added to it.
The results of this experiment showed that cooling rates should be modulated according to physiological characteristics of cryoprotectives; and a combination of cell penetrating and non-penetrating cryoprotectives had more synergistic effects in preserving rabbit spermatozoa than penetrating or non-penetrating cryoprotectives alone was used.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|